Transfection Reagents
Lonza Walkersville SE Cell Line 4D-Nucleofector X Kit L
The SE Cell Line 4D-Nucleofector X Kit L is one of our three 4D-Nucleofector Kits that is suited for transfecting difficult-to-transfect cell lines in the 100 µL Nucleocuvette Vessel format when working with the 4D-Nucleofector X Unit.Benefits:High transfection efficiencies in difficult-to-transfect cell linesCells maintain functionality post transfectionConditions are transferable between 4D-Nucleofector System (including 4D-Nucleofector including LV Unit), 96-well Shuttle System and 384-well Nucleofector System Applications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cellsLow throughput applicationsContent:2.25 mL SE Cell Line Nucleofector Solution0.5 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)24 x 100 µL Nucleocuvette Vessel,24 x Single use pipettes
HORIZON DISCOVERY BIOSCIENCES LIMITED DHARMAFECT4 TRANSFECTION,.75ML
DharmaFECT 4 Transfection Reagent0.75ml
Lonza Walkersville SG Cell Line 4D-Nucleofector X Kit S
The SG Cell Line 4D-Nucleofector X Kit S is one of our three 4D-Nucleofector Kits that is suited for transfecting difficult-to-transfect cell lines in the 20 µL 16-well Nucleocuvette Strip format when working with the 4D-Nucleofector X Unit.Benefits:High transfection efficiencies in difficult-to-transfect cell linesCells maintain functionality post transfectionConditions are transferable between 4D-Nucleofector System, 96-well Shuttle System and 384-well Nucleofector SystemApplications:Transfection of low cell numbers in the range of 5 x 10⁴ to 1 x 10⁶ cells Medium throughput applicationsOptimization of transfection conditions, e.g. substrate titrationsContent:1 x 0.675 mL SG Cell Line Nucleofector Solution1 x 0.15 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)2 x 16-well Nucleocuvette Strips (20 μl)
Lonza Walkersville Basic Nucleofector Kit for Primary Mammalian Epithelial Cells
In addition to various cell type-specific kits for primary epithelial cells, we also provide a cell group-specific Basic Nucleofector Kit. This kit is suited to determine the optimal transfection conditions for any mammalian epithelial cell type that lacks a cell type specific Optimized Protocol for the Nucleofector 2b Device (or older generations I or II).Benefits:Optimization can be performed within 1 experiment Detailed protocol guiding through the optimization procedure Optional result fine tuning with help of our Scientific Support Team Transfection efficiencies up to 83%Viabilities up to 98%Applications:Kit suited for epithelial cells from different mammalian species and various organsAlready tested for renal proximal tubular epithelial cells (RPTEC) human prostate epithelial cells (hPrEc) and human small airway epithelial cells (SAEC)
Lonza Walkersville Cell Line Optimization Nucleofector Kit for Nucleofector Device
The Cell Line Optimization Nucleofector Kit is the ideal tool to determine the optimal transfection conditions for any difficult-to-transfect cell line that lacks an Optimized Protocol for the Nucleofector 2b Device (or older generations I or II). It enables you to conveniently determine the optimal Nucleofection condition of your cell line of interest within one experiment. The kit contains two different Cell Line Nucleofector Solutions, V and L, each of which should be tested in combination with seven different Nucleofector Programs. It is suitable for 18 optimization reactions. Fine-tuning for optimal results can then be performed together with our Scientific Support Team.Benefits:Efficient transfection of virtually any difficult-to-transfect cell lineSimple and rapid optimization completed within just one experimentApplications:Optimization of Nucleofection conditions of virtually any difficult-to-transfect cell line with the Nucleofector II/2b Device
Lonza Walkersville P3 Primary Cell 96-well Nucleofector Kit
The P3 Primary Cell 96-well Nucleofector Kit is one of our five kits suited for medium-throughput transfection of primary cells when working with the 4D-Nucleofector 96-well Unit.Benefits:High transfection efficiencies for primary cellsCompatible with liquid handling systems for automated high-throughput electroporationConditions are transferable between 4D-Nucleofector System, 4D-Nucleofector 96-well Unit and 384-well Nucleofector SystemApplications:Transfection of low cell numbers in the range of 5 x 10⁴ to 1 x 10⁶ cells High-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR librariesContent:2.25 mL P3 Primary Cell Nucleofector Solution0.5 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)1 x 96-well Nucleocuvette Plate (20 μl)
Andwin Scientific JETPRIME DNA/SIRNA RGNT 5ML
The jetPRIME DNA and siRNA Transfection Reagent is made to guarantee high DNA transfection efficiency and excellent gene silencing. This product is very cost-effective as it uses less reagent and DNA and provides more gentle transfections in comparison to other competitors. It can be used with no difficulty and with serum and antibiotics. This can produce 15 reactions in 6-well plates and has a buffer volume of 5 mL. It is sold individually.
OriGene Lentiviral Packaging Kits, 10 reactions
Lenti-vpak packaging kit - packaging plasmids and transfection reagent.
FUGENT LLC FuGENE SI Transfection Reagent, 1.0mL
FuGENE SI is a novel, multicomponent transfection reagent for high efficiency, and low toxicity delivery of siRNA, miRNA, and other short RNAs into common and difficult-to-transfect eukaryotic cells. FuGENE SI enables researchers to obtain superior gene silencing and knockdown efficiencies while utilizing lower amounts of siRNAs. The simple, easy-to-use protocol with minimal optimization allows users to free up valuable lab time. Compatible with all commercially available and lab-synthesized siRNAs and miRNAs. See the Unseen with FuGENE SI.
Lonza Walkersville Human T Cell Nucleofector Kit
The Nucleofector 2b System (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. This Nucleofector Kit is the optimal kit for efficient transfection of primary human T cells (unstimulated or pre-stimulated).The cell type-specific Optimized Protocol is available in our knowledge database. In this Optimized Protocols the best Nucleofection Conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution for Human T Cells0.5 mL Supplement 130 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL), 25 Single use pipettes
HORIZON DISCOVERY BIOSCIENCES LIMITED DHARMAFECT 2 TRANSFECTION REAG
NC1361801 DHARMAFECT 2 TRANSFECTION REAG