Transfection Reagents
Lonza Walkersville Human T Cell Nucleofector Kit
The Nucleofector 2b System (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. This Nucleofector Kit is the optimal kit for efficient transfection of primary human T cells (unstimulated or pre-stimulated).The cell type-specific Optimized Protocol is available in our knowledge database. In this Optimized Protocols the best Nucleofection Conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution for Human T Cells0.5 mL Supplement 130 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL), 25 Single use pipettes
Bioland Scientific LLC BioT Transfection Reagent, 1 mL, lipid-based, non-toxic, broad range cell compatibility
BioT Transfection Reagent, 1 mL, lipid-based, non-toxic, broad range cell compatibility
Lonza Walkersville SG Cell Line 4D-Nucleofector X Kit L
The SG Cell Line 4D-Nucleofector X Kit L is one of our three 4D-Nucleofector Kits that is suited for transfecting difficult-to-transfect cell lines in the 100 µL Nucleocuvette Vessel format when working with the 4D-Nucleofector X Unit.Benefits:High transfection efficiencies in difficult-to-transfect cell linesCells maintain functionality post transfectionConditions are transferable between 4D-Nucleofector System, 96-well Shuttle System and 384-well Nucleofector SystemApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cellsLow throughput applicationsContent:2.25 mL SG Cell Line Nucleofector Solution0.5 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)24 x 100 µL Nucleocuvette Vessel24 x Single use pipettes
BIOLINK LABORATORIES LLC FECTOPRO TRANSFEC REAGENT 10ML
+10mL FectoPRO boosterReagent:FectroPRO®Molecule Delivered:DNAApplications:BioproductionCell Types:Suspension CHO, HEK-293, and derivativesNumber of Transfections:1mL of FectoPRO® is sufficient to transfect 800mL to more than 1L of cell cultureStorage:5°C ± 3°C, stable for 1 to 2 years when stored appropriatelyProvided with:FectoPRO® Booster
Lonza Walkersville Cell Line Optimization 4D-Nucleofector X Kit
The Cell Line Optimization 4D-Nucleofector X Kit is the ideal tool to conveniently and rapidly determine Nucleofection conditions for cell lines lacking an Optimized Protocol for the 4D-Nucleofector X Unit.Benefits:Convenient and rapid determination of optimal Nucleofection conditions for a broad range of primary cells within one experimentOptimal Nucleofection conditions determined in the 16-well Nucleocuvette Strips are transferable to the 100 μL single Nucleocuvette in the 4D-Nucleofector X UnitConditions are transferable between 4D Nucleofector System, 96-well Shuttle System and 384-well Nucleofector SystemApplications:Determination of Nucleofection conditions for cell lines lacking an Optimized Protocol for the 4D-Nucleofector X UnitContent:0.675 mL Nucleofector Solution SE0.675 mL Nucleofector Solution SF0.675 mL Nucleofector Solution SG3 x 0.15 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0) 4 x 20 µL 16-well Nucleocuvette Strips
LGC GENOMICS LLC (Lucigen) TransforMax EC100D pir-116 Electrocompetent E. coli, 10 RxnsPerform plasmid rescue for transposition mutants created with the EZ-Tn5 Transposome Kits.
These cells are used for rescue cloning of DNA transposed using the EZ-Tn5™
Sigma Aldrich Fine Chemicals Biosciences X-tremeGENE HP DNA Transfection Reagent, 0.4 mL
X-tremeGENE HP DNA Transfection Reagent is a multi-component reagent that forms a complex with DNA , then transports the complex into animal or insect cells.
Takara Bio XFECT TRANSFECTION REAGENT
Xfect Transfection reagent creates bio-degradable nanoparticles for superior transfection efficiency of plasmid DNA into mammalian cells.